Oxidation of Methionine Residues in Polypeptide Ions Via Gas-Phase Ion/Ion Chemistry
نویسندگان
چکیده
منابع مشابه
Selective Tuning of Elastin-like Polypeptide Properties via Methionine Oxidation.
We have designed and prepared a recombinant elastin-like polypeptide (ELP) containing precisely positioned methionine residues, and performed the selective and complete oxidation of its methionine thioether groups to both sulfoxide and sulfone derivatives. Since these oxidation reactions substantially increase methionine residue polarity, they were found to be a useful means to precisely adjust...
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A simple assay for the detection of 35S-labeled methionine sulfoxide residues in proteins is described. The assay, which is based on the ability of CNBr to react with methionine but not with methionine sulfoxide, requires the prelabeling of cellular proteins with [35S]methionine. The assay was used to study the extent of methionine oxidation in newly synthesized proteins of both activated and q...
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The mechanism of the low temperature oxidation of gaseous acetaldehyde was investigated in the temperature range of 1 50-400?°C. The minor, intermediate and major products were identified and measured quantitatively by sampling directly into the ionization chamber of an MS10-C2 mass spectrometer from the reactor. The formation of H2O, CO, CO2, HCOOH, H2, HCHO, CH3COOH and CH3OH as the major pro...
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Collision cross section measurements and molecular modeling calculations have been combined to extract average volumes of amino acid residues in gas-phase peptide ions. The approach uses cross sections for a series of 103 lysine-terminated peptide ions having the general form [XxxnLys+H] (where Xxx is any amino acid except Lys, Arg, His, and Cys, and n ) 4 to 8). The results show that average v...
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ژورنال
عنوان ژورنال: Journal of The American Society for Mass Spectrometry
سال: 2014
ISSN: 1044-0305,1879-1123
DOI: 10.1007/s13361-014-0861-8